investigate the possibility of sex determination of rainbow trout (oncorhynchus mykiss) using molecular markers
نویسندگان
چکیده
the study investigates the possibility of sex determination of rainbow trout (oncorhynchus mykiss)using molecular screen for products monosex of populations. 35 caudal fin tissue samples wereobtained from karaj rainbow trout mature population and then genomic dna was extracted andmarker loci amplified. polymerase chain reaction (pcr) was setup based on two omyfa andomyfatu markers for amplification of sex linked markers in rainbow trout. finally, the populationwas screened based on presence of a specific band for both markers. result of this expriments showedthat presence of omyfa and omyfatu markers in males were %100 and %55 respectively. acordingto the result omyfatu is proper marker for screen of karaj trout population.
منابع مشابه
Rainbow Trout, Oncorhynchus mykiss
Rainbow trout are classified as Oncorhynchus mykiss, and as such belong to the same genus as Pacific salmon, and to the family Salmonidae, which includes Atlantic salmon (Salmo salar), various trout (Salvelinus sp.), Arctic char (Salvelinus alpinus), Arctic grayling (Thymallus arcticus) and whitefish (Coregonus sp.). Rainbow trout are native to areas around the North Pacific Ocean, from souther...
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متن کاملGenetic variability of rainbow trout (Oncorhynchus mykiss) cultured in Iran using molecular RAPD markers
In the present study we evaluated the amount and distribution of genetic variation by using RAPD marker variation of 12 markers loci in three broodstock groups of rainbow trout. A total of 47 polymorphic bands were observed in Iranian strain, average number of bands was 10 and average number of polymorphic bands per primer was 3.92. The total detected bands in rainbow trout strain originated fr...
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It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a variety of species of fish stimulates the synthesis of, approximately, an equal amount of protein. Although synthesis of protein may account for as much as 40 % of the whole-animal oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized proteins are retained as growth (Houlihan et al. 1988; Ca...
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عنوان ژورنال:
شیلاتجلد ۶۸، شماره ۱، صفحات ۱-۱۱
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